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Image Search Results
Journal: Frontiers in Pharmacology
Article Title: Hemoglobin α-derived peptides VD-hemopressin (α) and RVD-hemopressin (α) are involved in electroacupuncture inhibition of chronic pain
doi: 10.3389/fphar.2024.1439448
Figure Lengend Snippet: Quantitative analysis of the protein level of 26S proteasome (PSMD2) in the PAG tissues. (A) The representative gel image shows the protein level of the PSMD2 in PAG tissues obtained from control (CON), KOA, and KOA treated with EA. β-actin was used as a loading control. The protein band at 100 kDa corresponds to the PSMD2. (B) Summary data show the effect of KOA and EA on the protein level of the PSMD2 in PAG tissues. Data are expressed as means ± SEM (n = 4 mice in each group). *** p < 0.001.
Article Snippet: MG132 (Z-Leu-Leu-Leu-al), a
Techniques: Control
Journal: Frontiers in Pharmacology
Article Title: Hemoglobin α-derived peptides VD-hemopressin (α) and RVD-hemopressin (α) are involved in electroacupuncture inhibition of chronic pain
doi: 10.3389/fphar.2024.1439448
Figure Lengend Snippet: Effects of the 26S proteasome inhibitor MG132 on EA analgesia and RVD-hemopressin (α) concentration in the PAG. (A, B) Time course of the effect of the 26S proteasome inhibitor MG132 on tactile and thermal withdrawal thresholds of EA mice. EA was administered for 30 min, once every other day for 4 weeks, starting from 2 days after the MIA injection. The 26S proteasome inhibitor MG132 (4 μg) was microinjected into the vlPAG 30 min before EA starting from 18 days after MIA injection, once every other day for five times, as indicated by the black arrow. Data are expressed as means ± SEM (n = 8–11 mice in each group). * p < 0.05, compared with the KOA group; # p < 0.05, compared with the 10% DMSO + EA group. (C) Summary data show the concentration of RVD-hemopressin (α) in the PAG. Data are expressed as means ± SEM (n = 4–6 mice in each group). * p < 0.001.
Article Snippet: MG132 (Z-Leu-Leu-Leu-al), a
Techniques: Concentration Assay, Injection
Journal: Frontiers in Pharmacology
Article Title: Hemoglobin α-derived peptides VD-hemopressin (α) and RVD-hemopressin (α) are involved in electroacupuncture inhibition of chronic pain
doi: 10.3389/fphar.2024.1439448
Figure Lengend Snippet: Effects of the 26S proteasome inhibitor MG132 on hemoglobin α-chain expression in the PAG. (A) The representative gel image shows the protein level of hemoglobin α-chain in PAG tissues obtained from CON, KOA, 10% DMSO + EA, and MG132 + EA groups. β-actin was used as a loading control. The protein band at 14 kDa corresponds to the hemoglobin α-chain. (B) Summary data show the effect of 26S proteasome inhibitor MG132 on the protein level of the hemoglobin α-chain in PAG tissues. EA was administered for 30 min, once every other day for 4 weeks, starting from 2 days after MIA injection. The 26S proteasome inhibitor MG132 (4 μg) was microinjected into the vlPAG 30 min before EA starting from 18 days after MIA injection, once every other day for five times. Data are expressed as means ± SEM (n = 3 mice in each group). * p < 0.05.
Article Snippet: MG132 (Z-Leu-Leu-Leu-al), a
Techniques: Expressing, Control, Injection